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A wide range of nutritional tests have been performed on Rosita EVCLO. The “Nutritional Analysis” presented represent only a small fraction of this tests which include vitamins, and specialist chemistry testing.

 

1. Nutrient Results

2. Purity

3. Oxidation byproducts

4. Hydrolytic Rancidity Measures

5. Microbiological Testing

6. Nutrition Facts

7. Shelf-Life Study Results

 

 

 

1. Nutrient Results

The concentration of fat-soluble nutrients depends on several biological factors, including the size of the fish and the amount of oil contained in the liver. There are also seasonal variations related to the spawning cycle of the fish, climatic conditions, plankton, and other conditions affecting the codfish. Because Rosita EVCLO is a completely unrefined & unprocessed wholefood, the concentration of nutrients will ALWAYS vary from batch to batch.

The Nutrient Results (fat-soluble vitamins, sterols & fatty acids) shown below represent typical values and ranges which have been obtained from analytical testing at the following institutes:

  • NIFES (National Institute of Nutrition and Seafood Research )[20/12/2010]
  • ALS Food & Pharmaceutical (United Kingdom) [10/10/2012]
  • Eurofins Food Testing [19/02/2013]
  • Nutrition Analytical Service (NAS), Institute of Aquaculture, University of Stirling, Scotland, United Kingdom [15/11/2013]. The head of NAS, Professor Gordon Bell, has over 30 years of experience in fish nutrition and lipid and nutrition related analysis and has published more than 184 peer-reviewed papers on fish nutrition, fatty acids, lipid soluble nutrients and antioxidants. The Chief Analyst, James Dick, provides over 25 years of experience in lipid methodology and is responsible for Quality Control within the Nutrition Group, the development of chromatographic techniques and is trained in all aspects of complex lipid analysis including HPLC, GC, GC/MS and GC/MS/MS.
  • Extra virgin oil was also tested [as far back as: 15.07.2011] by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products. Project no (C3937).

 

VITAMIN E (ALPHA-TOCOPHEROL) ANALYSIS

 SAMPLE ID

 mg/teaspoon

 Wild & Raw EVCLO

3.7-10

Above values calculated from analyses performed at a number of European testing institutes
(named above), and each testing institute analyses has been performed in duplicate.

 

VITAMIN A (RETINOL) ANALYSIS

 SAMPLE ID

iu/teaspoon

 Wild & Raw EVCLO

3000-5000

Above values calculated from analyses performed at a number of European testing institutes
(named above), and each testing institute analyses has been performed in duplicate.

 

VITAMIN A2 (3,4-DEHYDRORETINOL) ANALYSIS

 SAMPLE ID

 mg/kg

 Wild & Raw EVCLO

> = 0.1

> = Greater Than or Equal To.
 
Above values calculated from analyses performed at a number of European testing institutes
(named above), and each testing institute analyses has been performed in duplicate.

 

VITAMIN D3 (CHOLECALCIFEROL) ANALYSIS#

 SAMPLE ID

 iu/teaspoon

 Wild & Raw EVCLO

400-500

Above values calculated from analyses performed at a number of European testing institutes
(named above), and each testing institute analyses has been performed in duplicate.
 
#The vitamin D in Wild & Raw EVCLO is the same form of vitamin D that your body produces when
exposed to sunlight and the only form found in significant quantities in unrefined fish liver oils. 

 

RATIO VITAMINS A:D

 SAMPLE ID

 ratio

 Wild & Raw EVCLO

Typically less than 10:1

 

 STEROL CONTENT

SAMPLE ID

mg/Kg

Wild & Raw EVCLO

 

CHOLESTEROL

4044-5245

BRASSICASTEROL (PHYTOSTEROL)

40.4

Rest to follow

Above values calculated from analyses performed at a number of European testing institutes
(named above), and each testing institute analyses has been performed in duplicate.

Select Fatty Acid Composition of Total Lipid from Wild & Raw EVCLO

Wild & Raw EVCLO contains a wide spectrum of fatty acids including omega 3, 4, 6, 7, 9, 11 etc.
The test data for selective fatty acids are given below. Levels of other fatty acids not shown are available on request.

Fatty acid

mg/teaspoon

20:5n-3 (EPA)

510

22:6n-3 (DHA)

700

18:1n-9 (Oleic acid)

1,200

20:1n-9 (Gadoleic acid)*

660

22:1n-11 (Cetoleic acid)*

Fatty acids, Sum of Omega 6 Calc.

Fatty acids, Sum of Omega 3 Calc.

Unidentified fatty acids

375

160

1,460

240

ratio

DHA/EPA

1:1 – 1.3:1

Omega 6/Omega 3 Ratio

0.11

 
*Gadoleic acid [which was first noted in cod liver oil] and Cetoleic acid are present in higher amounts in codfish from Northern hemisphere and high latitude waters. These fatty acids are therefore found in higher quantities in genuine cod liver oil originating from the Northern hemisphere.
 
*Cetoleic acid is also usually present in lower proportions than Gadoleic acid.

This ratio is a good indicator of genuine Northern hemisphere cod liver oil.

 

 

2. Purity

 

Cod Liver Oil has been tested for purity by leading European institutes and meets all the stringent European requirements.

HEAVY METAL CONTENT

[ALS Food & Pharmaceutical (UK): 10/10/2012]

 

SAMPLE ID

Wild & Raw EVCLO

MERCURY

n.d

CADMIUM

n.d

LEAD

n.d

ARSENIC

1.4 ppm*

ppm - parts per million

n.d - not detected

*organic (non-toxic) form

 

HEAVY METAL ANALYSIS

[BioDetection Systems b.v. (BDS): 19/03/2013]

 

SAMPLE ID

Wild & Raw EVCLO

MERCURY

n.d

CADMIUM

n.d

LEAD

n.d

ARSENIC

1.8 ppm*

ppm - parts per million

n.d - not detected (the concentration of the analyte is below the limit of quantification).

*organic (non-toxic) form

 

ORGANIC CONTAMINANTS [Dioxins and PCBs, Benzo(a)pyrene]

Certificate of Analysis from:

  • ALS Food & Pharmaceutical (UK) [10/10/2012]
  • BioDetection Systems b.v. (BDS) [19/03/2013]

Cod liver oil has been analysed in triplicate and each time gives a total WHO-PCDD/F-PCB-TEQ lower than the strict European limits and well within them. The values for the oil are in line with QC parameters.

 

A. POLYAROMATIC HYDROCARBONS

 

 SAMPLE ID

 BENZO(A)PYRENE
(mg/kg)

 Wild & Raw EVCLO

 <0.001#

#the concentration of the analyte is below the limit of quantification

Above value calculated from analyses performed in duplicate.

 

B. DIOXINS and PCBs

  • Sum of dioxins (WHO-PCDD/F-TEQ): 0.20 ng/kg#
  • Sum of dioxins and dioxin-like PCBS (WHO-PCDD/F-PCB-TEQ): 0.899 ng/kg#

#Maximum levels set by the European Commission for thesum of dioxins (WHO-PCDD/F-TEQ) for marine oils (fish body oil, fish liver oil and oils of other marine organisms intended for human consumption): 1.75 ng/kg fat

#Maximum levels set by the European Commission for thesum of dioxins and dioxin like PCBs (WHO-PCDD/F-PCB-TEQ) for marine oils (fish body oil, fish liver oil and oils of other marine organisms intended for human consumption): 6.0 ng/kg fat

#Dioxins (sum of polychlorinated dibenzo-para-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs), expressed as World Health Organisation (WHO) toxic equivalent using the WHO-toxic equivalency factors (WHO-TEFs)) and sum of dioxins and dioxin-like PCBs (sum of PCDDs, PCDFs and polychlorinated biphenyls (PCBs), expressed as WHO toxic equivalent using the WHO-TEFs). WHO-TEFs for human risk assessment based on the conclusions of the World Health Organization (WHO) – International Programme on Chemical Safety (IPCS) expert meeting which was held in Geneva in June 2005 (Martin van den Berg et al., The 2005 World Health Organization Re-evaluation of Human and Mammalian Toxic Equivalency Factors for Dioxins and Dioxin-like Compounds. Toxicological Sciences 93(2), 223–241 (2006))

 

C. BDS DR CALUX technique: another screening method for dioxins and dioxin-like PCBs

 

SAMPLE ID

Method

Parameter

Result

Unit

Wild & Raw EVCLO

DR CALUX

PCDD/PCDF and dl-PCBs (total BEQ; semi)

compliant (1.7)

ng BEQ/kg product

 

The DR CALUX analysis is done according to p-bds-051.

All DR CALUX analysis results comply with EU requirements as indicated in COMMISSION REGULATION (EC) No 278/2012 (amending Regulation (EC) No 152/2009 as regards the determination of the levels of dioxins and polychlorinated biphenyls; feedstuffs).

 

 3. Oxidation byproducts

 

TOTOX ANALYSIS

Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products [11/11/2013]. Extra virgin oil was also tested as far back as 15.07.2011

SAMPLE ID

PEROXIDE VALUE *

ANISIDINE VALUE *  

TOTOX *

Wild & Raw EVCLO

4.8

2.2 

11.8

PV expressed as Meq/Kg

AV expressed as anisidine value units

TOTOX = 2PV+AV

 

TOTOX ANALYSIS

Nutrition Analytical Service, Institute of Aquaculture,
University of Stirling, Scotland, United Kingdom [15/11/2013]

SAMPLE ID

PEROXIDE VALUE *

ANISIDINE VALUE *  

TOTOX *

Wild & Raw EVCLO

 0.38

 0

0.76

PV expressed as Meq/Kg

AV expressed as anisidine value units

TOTOX = 2PV+AV

 

TOTOX ANALYSIS

Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products [01/02/2014]. Extra virgin oil was also tested as far back as 15.07.2011

SAMPLE ID

PEROXIDE VALUE *

ANISIDINE VALUE *  

TOTOX *

Wild & Raw EVCLO

2.9

6.6

12.4

PV expressed as Meq/Kg

AV expressed as anisidine value units

TOTOX = 2PV+AV

*Peroxide value:
NSF limit = < 10 Meq/Kg
CRN limit = 5 Meq/Kg
GOED limit = 5 Meq/Kg
Stringent European Pharmacopoeia Commision max = 5 Meq/Kg
*Anisidine value:
NSF limit = < 20
CRN limit = 20
GOED limit = 20
Stringent European Pharmacopoeia Commision max = 10
*TOTOX:
NSF limit = < 26 Meq/Kg
CRN limit = 26 Meq/Kg
GOED limit = 26 Meq/Kg
Stringent European Pharmacopoeia Commision max = 20 Meq/Kg
NSF: National Sanitation Foundation International
CRN: Council for Responsible Nutrition
GOED: Global Organization for EPA and DHA Omega-3s

 

TBAR ANALYSIS

Nutrition Analytical Service, Institute of Aquaculture,
University of Stirling, Scotland, United Kingdom [15/11/2013]

SAMPLE ID

TBARS (mg/kg)

Wild & Raw EVCLO

0.25

Above value calculated from analyses performed in duplicate.

TBARs are expressed as mg/kg for feeds and oil and nmole/g tissue for flesh or other tissues.

 

 4. Hydrolytic Rancidity Measures

 

[Extra virgin oil was also tested by the producer at ECO-Marine using Nofima (the Norwegian Institute of Food, Fisheries and Aquaculture Research) BioLab which specialises in marine raw materials and products: 01/02/2014]

% FREE FATTY ACID ANALYSIS
 

SAMPLE ID  

%FFA as Oleic

Wild & Raw EVCLO

0.6 

 
ACID VALUE
 

SAMPLE ID  

mg KOH/g

Wild & Raw EVCLO

1.2

 

 

5. Microbiological Testing

MICROBIOLOGICAL CONTENT

[ALS Food & Pharmaceutical (UK): 10/10/2012]

SAMPLE ID

cfu/ml

Wild & Raw EVCLO

 

Total Viable Count

<1

Coliforms (presumptive)

0

Escherichia coli (presumptive)

<1

Yeasts

<2

Moulds

<2

Salmonella sp. (ELISA)

Not detected / 25g

 

 

6. Nutrition Facts

 CLO-Bottle2-h500

Wild & Raw Extra-Virgin Cod Liver Oil: 150 ml
Suggested Serving Size: 1 teaspoon (5 ml) daily
Servings per Bottle: Approximately 30
Amount Per Serving:
Calories 45
Calories from Fat 45
% Daily Value*
Total Fat 4.5g
7.0%
Saturated Fat 0.9g
4.5%
Trans Fat 0g
0%
Cholesterol 18mg
6%
* Percent Daily Values are based on a caloric intake of 2,000 calories.
Does not contain: Gluten, yeast, milk derivatives, synthetic nutrients, artificial colours or flavours.

 

 7. Shelf-Life Study Results

 

[Performed by a third party, independent Norwegian laboratory].

  • Shelf-life of unopened bottles at a temperature of 0-4 °C is 12 months.
  • For storage at -20 °C the shelf-life is 2-3 times longer.
  • Shelf-life after the bottles are opened at 0-4 °C is 2-3 months.
  • Testing has shown that vitamins and other substances found in Rosita EVCLO are not affected by freezing and remain completely intact.
  • In summary, the oil must be kept dark and cold.

 

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